UNIT 11.1 Large Scale Sequencing
Published Online: 1 AUG 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Bioinformatics
How to Cite
Stein, L. 2003. Large Scale Sequencing. Current Protocols in Bioinformatics. 2:11.1:11.1.1–11.1.3.
- Published Online: 1 AUG 2003
- Published Print: MAY 2003
This is not the most recent version of the article. View current version (1 DEC 2011)
DNA sequencing and assembly is the Philosopher's Stone of bioinformatics, where the raw material of wet lab enzymatic chemistry is transformed into the bits and bytes of sequence information. The sequence information becomes the foundation for extensive downstream analyses. This unit provides a brief overview of the sequencing process. It describes several software programs typically used for base calling, assembling, and finishing sequence data such as STADEN, Phred, Phrap, and Consed.