UNIT 11.3 Generating a Genome Assembly with PCAP
Published Online: 1 OCT 2005
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Bioinformatics
How to Cite
Huang, X. and Yang, S.-P. 2005. Generating a Genome Assembly with PCAP. Current Protocols in Bioinformatics. 11:11.3:11.3.1–11.3.23.
- Published Online: 1 OCT 2005
- Published Print: SEP 2005
This unit describes how to use the Parallel Contig Assembly Program (PCAP) to assemble the data produced by a whole-genome shotgun sequencing project. We present a basic protocol for using PCAP on a multiprocessor computer in a 300-Mb genome assembly project. A support protocol to prepare input files for PCAP is also described. Another basic protocol for using PCAP on a distributed cluster of computers in a 3-Gb genome assembly project is presented, in addition to suggestions for understanding results from PCAP.
- Whole-Genome Shotgun Sequencing;
- Genome Assembly