UNIT 12.3 RNAi: Design and Analysis
Published Online: 1 SEP 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Bioinformatics
How to Cite
Sachidanandam, R. 2004. RNAi: Design and Analysis. Current Protocols in Bioinformatics. 6:12.3:12.3.1–12.3.10.
- Published Online: 1 SEP 2004
- Published Print: JUN 2004
RNAi is a novel genetic tool that is poised to revolutionize the study of genetics and gene networks. Use of this tool requires knowledge of the underlying mechanisms and application of this knowledge to the construction of a gene-silencing library. This unit discusses approaches to rational design of this library and its use in experiments. It focuses on the design algorithms developed so far and briefly discuss several issues that could lead to improvements in the current design algorithms.
- Oligo design;