UNIT 12.3 RNAi: Design and Analysis

  1. Ravi Sachidanandam

Published Online: 1 SEP 2004

DOI: 10.1002/0471250953.bi1203s6

Current Protocols in Bioinformatics

Current Protocols in Bioinformatics

How to Cite

Sachidanandam, R. 2004. RNAi: Design and Analysis. Current Protocols in Bioinformatics. 6:12.3:12.3.1–12.3.10.

Author Information

  1. Sachidanandam Lab, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

Publication History

  1. Published Online: 1 SEP 2004
  2. Published Print: JUN 2004


RNAi is a novel genetic tool that is poised to revolutionize the study of genetics and gene networks. Use of this tool requires knowledge of the underlying mechanisms and application of this knowledge to the construction of a gene-silencing library. This unit discusses approaches to rational design of this library and its use in experiments. It focuses on the design algorithms developed so far and briefly discuss several issues that could lead to improvements in the current design algorithms.


  • RNAi;
  • Oligo design;
  • siRNA;
  • miRNA;
  • shRNA