Unit

UNIT 13.1 Proteomics and the Analysis of Proteomic Data: An Overview of Current Protein-Profiling Technologies

  1. Erol E. Gulcicek1,
  2. Christopher M. Colangelo1,
  3. Walter McMurray1,
  4. Kathryn Stone1,
  5. Kenneth Williams1,
  6. Terence Wu1,
  7. Hongyu Zhao1,
  8. Heidi Spratt2,
  9. Alexander Kurosky2,
  10. Baolin Wu3

Published Online: 1 JUL 2005

DOI: 10.1002/0471250953.bi1301s10

Lab Protocol Title

Current Protocols in Bioinformatics

Current Protocols in Bioinformatics

Additional Information

How to Cite

Gulcicek, E. E., Colangelo, C. M., McMurray, W., Stone, K., Williams, K., Wu, T., Zhao, H., Spratt, H., Kurosky, A. and Wu, B. 2005. Proteomics and the Analysis of Proteomic Data: An Overview of Current Protein-Profiling Technologies. Current Protocols in Bioinformatics. 10:13.1:13.1.1–13.1.31.

Author Information

  1. 1

    Yale University, New Haven, Connecticut

  2. 2

    University of Texas Medical Branch, Galveston, Texas

  3. 3

    University of Minnesota, Minneapolis, Minnesota

Publication History

  1. Published Online: 1 JUL 2005
  2. Published Print: JUN 2005
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Abstract

In recent years, several proteomic methodologies have been developed that now make it possible to identify, characterize, and comparatively quantify the relative level of expression of hundreds of proteins that are coexpressed in a given cell type or tissue, or that are found in biological fluids such as serum. These advances have resulted from the integration of diverse scientific disciplines including molecular and cellular biology, protein/peptide chemistry, bioinformatics, analytical and bioanalytical chemistry, and the use of instrumental and software tools such as multidimensional electrophoretic and chromatographic separations and mass spectrometry. In this unit, some of the common protein-profiling technologies are reviewed, along with the accompanying data-analysis tools.

Keywords:

  • Proteomics;
  • Protein profiling;
  • protein analysis;
  • protein identification;
  • serum biomarkers;
  • computational tools;
  • database searches
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