UNIT 13.2 Finding Protein Sequences Using PROWL

  1. Ronald Beavis1,
  2. David Fenyö2

Published Online: 1 OCT 2004

DOI: 10.1002/0471250953.bi1302s7

Current Protocols in Bioinformatics

Current Protocols in Bioinformatics

How to Cite

Beavis, R. and Fenyö, D. 2004. Finding Protein Sequences Using PROWL. Current Protocols in Bioinformatics. 7:13.2:13.2.1–13.2.20.

Author Information

  1. 1

    Beavis Informatics Ltd., Winnipeg, Manitoba, Canada

  2. 2

    GE Healthcare, Piscataway, New Jersey

Publication History

  1. Published Online: 1 OCT 2004
  2. Published Print: SEP 2004


PROWL is a collection of tools for the identification of protein sequences, using input data derived from mass spectrometry. Experimental data from various types of mass spectrometers can be input directly into PROWL's component software. This unit presents protocols for several of the individual PROWL tools. Specifically, PepFrag allows for the analysis of a single spectrum derived from tandem mass spectrometry. GPM, on the other hand, provides for the analysis of multiple MS/MS spectra. An additional protocol introduces ProFound for analyzing a single spectrum of peptide mass fingerprinting data.