UNIT 13.3 Protein Identification Using Sorcerer 2 and SEQUEST
Published Online: 1 DEC 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Bioinformatics
How to Cite
Lundgren, D. H., Martinez, H., Wright, M. E. and Han, D. K. 2009. Protein Identification Using Sorcerer 2 and SEQUEST. Current Protocols in Bioinformatics. 28:13.3:13.3.1–13.3.21.
- Published Online: 1 DEC 2009
- Published Print: DEC 2009
Sage-N's Sorcerer 2 provides an integrated data analysis system for comprehensive protein identification and characterization. It runs on a proprietary version of SEQUESTR, the most widely used search engine for identifying proteins in complex mixtures. The protocol presented here describes the basic steps performed to process mass spectrometric data with Sorcerer 2 and how to analyze results using TPP and Scaffold. The unit also provides an overview of the SEQUESTR algorithm, along with Sorcerer-SEQUESTR enhancements, and a discussion of data filtering methods, important considerations in data interpretation, and additional resources that can be of assistance to users running Sorcerer and interpreting SEQUESTR results. Curr. Protoc. Bioinform. 28:13.3.1-13.3.21. © 2009 by John Wiley & Sons, Inc.
- post-translational modifications;
- false discovery rate;