Unit

UNIT 13.4 Validation of Tandem Mass Spectrometry Database Search Results Using DTASelect

  1. Daniel Cociorva,
  2. David L. Tabb,
  3. John R. Yates

Published Online: 1 JAN 2007

DOI: 10.1002/0471250953.bi1304s16

Current Protocols in Bioinformatics

Current Protocols in Bioinformatics

How to Cite

Cociorva, D., L. Tabb, D. and Yates, J. R. 2007. Validation of Tandem Mass Spectrometry Database Search Results Using DTASelect. Current Protocols in Bioinformatics. 16:13.4:13.4.1–13.4.14.

Author Information

  1. The Scripps Research Institute, La Jolla, California

Publication History

  1. Published Online: 1 JAN 2007
  2. Published Print: DEC 2006

Abstract

DTASelect provides a means by which complex SEQUEST results can be filtered, organized, and viewed. A single sample may produce tens of thousands of tandem mass spectra. Manually perusing and selecting SEQUEST matches among such a mass of data carries a risk of inconsistency. DTASelect allows the user to set complex criteria for acceptance or rejection of individual spectrum results. It also features rules for dealing with multiple, identical peptide matches and for removing proteins that are insufficiently evidenced. It provides its sorted and filtered summary as HTML and text documents for easy review and also offers several auxiliary reports. DTASelect is a powerful tool for automatic analysis of complex mixture tandem mass spectrometry.

Keywords:

  • tandem mass spectrometry;
  • database searching;
  • statistical validation;
  • data filtering.