Unit
UNIT 13.7 Using BiblioSpec for Creating and Searching Tandem MS Peptide Libraries
Published Online: 1 DEC 2007
DOI: 10.1002/0471250953.bi1307s20
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Bioinformatics
Additional Information
How to Cite
Frewen, B. and MacCoss, M. J. 2007. Using BiblioSpec for Creating and Searching Tandem MS Peptide Libraries. Current Protocols in Bioinformatics. 20:13.7:13.7.1–13.7.12.
Publication History
- Published Online: 1 DEC 2007
- Published Print: DEC 2007
- Abstract
- Article
- Figures
- Tables
- References
Abstract
BiblioSpec is a software package for creating and searching libraries of tandem MS peptide spectra. Library searching provides a quick method for making peptide-spectrum matches by comparing a query spectrum to a collection of reference spectra of known peptide sequence. Pre-assembled libraries for several model organisms can be used as the basis of a search or custom libraries can easily be assembled. The protocols in this unit describe installing BiblioSpec, searching libraries, and creating custom libraries. Curr. Protoc. Bioinform. 20:13.7.1-13.7.12. © 2007 by John Wiley & Sons, Inc.
Keywords:
- proteomics;
- peptide spectrum library;
- peptide identification
