UNIT 13.7 Using BiblioSpec for Creating and Searching Tandem MS Peptide Libraries

  1. Barbara Frewen,
  2. Michael J. MacCoss

Published Online: 1 DEC 2007

DOI: 10.1002/0471250953.bi1307s20

Current Protocols in Bioinformatics

Current Protocols in Bioinformatics

How to Cite

Frewen, B. and MacCoss, M. J. 2007. Using BiblioSpec for Creating and Searching Tandem MS Peptide Libraries. Current Protocols in Bioinformatics. 20:13.7:13.7.1–13.7.12.

Author Information

  1. University of Washington, Seattle, Washington

Publication History

  1. Published Online: 1 DEC 2007
  2. Published Print: DEC 2007


BiblioSpec is a software package for creating and searching libraries of tandem MS peptide spectra. Library searching provides a quick method for making peptide-spectrum matches by comparing a query spectrum to a collection of reference spectra of known peptide sequence. Pre-assembled libraries for several model organisms can be used as the basis of a search or custom libraries can easily be assembled. The protocols in this unit describe installing BiblioSpec, searching libraries, and creating custom libraries. Curr. Protoc. Bioinform. 20:13.7.1-13.7.12. © 2007 by John Wiley & Sons, Inc.


  • proteomics;
  • peptide spectrum library;
  • peptide identification