Unit
UNIT 13.10 De Novo Interpretation of Tandem Mass Spectra
Published Online: 1 MAR 2009
DOI: 10.1002/0471250953.bi1310s25
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Bioinformatics
Additional Information
How to Cite
Ma, B. and Lajoie, G. 2009. De Novo Interpretation of Tandem Mass Spectra. Current Protocols in Bioinformatics. 25:13.10:13.10.1–13.10.8.
Publication History
- Published Online: 1 MAR 2009
- Published Print: MAR 2009
- Abstract
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Abstract
De novo sequencing is an effective method for identifying unknown peptide sequences from their tandem mass spectra. This unit briefly introduces how this can be done manually. A protocol for using the PEAKS online software for automated de novo sequencing is described. Finally, we show how to use the PEAKS scores to validate the de novo sequencing results. Curr. Protoc. Bioinform. 25:13.10.1-13.10.8. © 2009 by John Wiley & Sons, Inc.
Keywords:
- de novo sequencing;
- tandem mass spectrometry;
- protein identification;
- peptide sequencing;
- PEAKS software
