UNIT 13.10 De Novo Interpretation of Tandem Mass Spectra
Published Online: 1 MAR 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Bioinformatics
How to Cite
Ma, B. and Lajoie, G. 2009. De Novo Interpretation of Tandem Mass Spectra. Current Protocols in Bioinformatics. 25:13.10:13.10.1–13.10.8.
- Published Online: 1 MAR 2009
- Published Print: MAR 2009
De novo sequencing is an effective method for identifying unknown peptide sequences from their tandem mass spectra. This unit briefly introduces how this can be done manually. A protocol for using the PEAKS online software for automated de novo sequencing is described. Finally, we show how to use the PEAKS scores to validate the de novo sequencing results. Curr. Protoc. Bioinform. 25:13.10.1-13.10.8. © 2009 by John Wiley & Sons, Inc.
- de novo sequencing;
- tandem mass spectrometry;
- protein identification;
- peptide sequencing;
- PEAKS software