UNIT 13.16 Using the scan-x Web Site to Predict Protein Post-Translational Modifications

  1. Michael F. Chou1,
  2. Daniel Schwartz2

Published Online: 1 DEC 2011

DOI: 10.1002/0471250953.bi1316s36

Current Protocols in Bioinformatics

Current Protocols in Bioinformatics

How to Cite

Chou, M. F. and Schwartz, D. 2011. Using the scan-x Web Site to Predict Protein Post-Translational Modifications. Current Protocols in Bioinformatics. 36:13.16:13.16.1–13.16.8.

Author Information

  1. 1

    Department of Genetics, Harvard Medical School, Boston, Massachusetts

  2. 2

    Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut

Publication History

  1. Published Online: 1 DEC 2011
  2. Published Print: DEC 2011


The recent plethora of proteomic mass spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post-translational modification. We describe a protocol to use the scan-x Web site to view predicted acetylation sites in the human proteome and predicted phosphorylation sites in the human, mouse, fly, and yeast proteomes with high specificity. This tool is accessible from virtually any computer with a Web browser. The only requirement is a means of searching for a protein of interest in one of the represented organisms. Curr. Protoc. Bioinform. 36:13.16.1-13.16.8. © 2011 by John Wiley & Sons, Inc.


  • acetylation;
  • phosphorylation;
  • post-translational modification (PTM);
  • scan-x;
  • motif-x;
  • mass spectrometry;
  • proteomics