UNIT 14.11 LC-MS Data Processing with MAVEN: A Metabolomic Analysis and Visualization Engine
Published Online: 1 MAR 2012
Copyright © 2012 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Bioinformatics
How to Cite
Clasquin, M. F., Melamud, E. and Rabinowitz, J. D. 2012. LC-MS Data Processing with MAVEN: A Metabolomic Analysis and Visualization Engine. Current Protocols in Bioinformatics. 37:14.11:14.11.1–14.11.23.
- Published Online: 1 MAR 2012
- Published Print: MAR 2012
MAVEN is an open-source software program for interactive processing of LC-MS-based metabolomics data. MAVEN enables rapid and reliable metabolite quantitation from multiple reaction monitoring data or high-resolution full-scan mass spectrometry data. It automatically detects and reports peak intensities for isotope-labeled metabolites. Menu-driven, click-based navigation allows visualization of raw and analyzed data. Here we provide a User Guide for MAVEN. Step-by-step instructions are provided for data import, peak alignment across samples, identification of metabolites that differ strongly between biological conditions, quantitation and visualization of isotope-labeling patterns, and export of tables of metabolite-specific peak intensities. Together, these instructions describe a workflow that allows efficient processing of raw LC-MS data into a form ready for biological analysis. Curr. Protoc. Bioinform. 37:14.11.1-14.11.23. © 2012 by John Wiley & Sons, Inc.
- liquid chromatography-mass spectrometry;
- pathway visualization and mapping;
- stable isotope labeling;
- metabolic flux analysis;
- kinetic flux profiling