Standard Article

Image Analysis of Microorganisms

  1. John R. Lawrence1,
  2. Darren R. Korber2,
  3. Gideon M. Wolfaardt3

Published Online: 15 JAN 2003

DOI: 10.1002/0471263397.env042

Encyclopedia of Environmental Microbiology

Encyclopedia of Environmental Microbiology

How to Cite

Lawrence, J. R., Korber, D. R. and Wolfaardt, G. M. 2003. Image Analysis of Microorganisms. Encyclopedia of Environmental Microbiology. .

Author Information

  1. 1

    National Water Research Institute, Saskatoon, Saskatchewan, Canada

  2. 2

    University of Saskatchewan, Saskatoon, Saskatchewan, Canada

  3. 3

    University of Stellenbosch, Matieland, South Africa

Publication History

  1. Published Online: 15 JAN 2003


  • Obtaining an Image

  • Fluorescent Labels for Epifluorescence, CLSM and 2-PLSM

  • Video/Digital Camera Selection

  • Image Analysis/Processing Systems

  • The Basics of Image Acquisition

  • Presegmentation Processing Steps

  • Segmentation/Thresholding

  • Enhancement of Binary Images

  • Difference Imagery

  • Field and Object Parameters

  • Calibration

  • Replication and Sampling Intensity

  • Applications of Image Processing and Analyses


  • confocal laser scanning microscopy (CLSM);
  • epifluorescence microscopy;
  • image analysis;
  • methodology