Chapter 11. Transient Phases of Enzymatic Reactions
- Alejandro G. Marangoni
Published Online: 13 MAR 2003
Copyright © 2003 John Wiley & Sons, Inc.
Enzyme Kinetics: A Modern Approach
How to Cite
Marangoni, A. G. (2002) Transient Phases of Enzymatic Reactions, in Enzyme Kinetics: A Modern Approach (ed A. G. Marangoni), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471267295.ch11
Department of Food Science, University of Guelph, Canada
- Published Online: 13 MAR 2003
- Published Print: 1 NOV 2002
Print ISBN: 9780471159858
Online ISBN: 9780471267294
- pre steady-state;
In this chapter, the kinetics of enzyme-catalyzed reactions in the pre-steady, or transient region are discussed. The principles behind rapid reaction techniques, including continuous and quick-quench flow techniques, are presented. A general kinetic model for pre-steady state reaction mechanisms is developed. Relaxation techniques used in the determination of rate constants are also discussed.