Chapter 11. Transient Phases of Enzymatic Reactions

  1. Alejandro G. Marangoni
  1. Alejandro G. Marangoni

Published Online: 13 MAR 2003

DOI: 10.1002/0471267295.ch11

Enzyme Kinetics: A Modern Approach

Enzyme Kinetics: A Modern Approach

How to Cite

Marangoni, A. G. (2002) Transient Phases of Enzymatic Reactions, in Enzyme Kinetics: A Modern Approach (ed A. G. Marangoni), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471267295.ch11

Editor Information

  1. Department of Food Science, University of Guelph, Canada

Author Information

  1. Department of Food Science, University of Guelph, Canada

Publication History

  1. Published Online: 13 MAR 2003
  2. Published Print: 1 NOV 2002

ISBN Information

Print ISBN: 9780471159858

Online ISBN: 9780471267294

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Keywords:

  • pre steady-state;
  • transient;
  • rapid;
  • quick-quench;
  • continuous-flow;
  • relaxation

Summary

In this chapter, the kinetics of enzyme-catalyzed reactions in the pre-steady, or transient region are discussed. The principles behind rapid reaction techniques, including continuous and quick-quench flow techniques, are presented. A general kinetic model for pre-steady state reaction mechanisms is developed. Relaxation techniques used in the determination of rate constants are also discussed.