Chapter 13. Mechanism-Based Inhibition
- Alejandro G. Marangoni
Published Online: 13 MAR 2003
DOI: 10.1002/0471267295.ch13
Copyright © 2003 John Wiley & Sons, Inc.
Book Title
Enzyme Kinetics: A Modern Approach
Additional Information
How to Cite
Copp, L. J. (2003) Mechanism-Based Inhibition, in Enzyme Kinetics: A Modern Approach (ed A. G. Marangoni), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471267295.ch13
Editor Information
Department of Food Science, University of Guelph, Canada
Publication History
- Published Online: 13 MAR 2003
- Published Print: 1 NOV 2002
ISBN Information
Print ISBN: 9780471159858
Online ISBN: 9780471267294
- Summary
- Chapter
Keywords:
- catalytic inhibition;
- suicide inhibition;
- irreversible inhibition;
- inactivation;
- alternate substrate inhibition;
- acyl-enzyme
Summary
This chapter describes both reversible and irreversible mechanism-based inhibition of enzymes. Mechanism-based inhibitors require the inhibitor to be processed catalytically by the enzyme for inhibitory activity. Methods for determining kinetic parameters, such as rates of inactivation and reactivation, the partition ratio and dissociation constants for inhibition or inactivation are described. Suggestions for the determination of products and verifying the nature of the inhibition are also discussed. The various methods are illustrated with several examples of Human Leukocyte mechanism-based inhibitors.