Chapter 14. Putting Kinetic Principles into Practice
- Alejandro G. Marangoni
Published Online: 13 MAR 2003
DOI: 10.1002/0471267295.ch14
Copyright © 2003 John Wiley & Sons, Inc.
Book Title
Enzyme Kinetics: A Modern Approach
Additional Information
How to Cite
Parkin, K. L. (2003) Putting Kinetic Principles into Practice, in Enzyme Kinetics: A Modern Approach (ed A. G. Marangoni), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471267295.ch14
Editor Information
Department of Food Science, University of Guelph, Canada
Publication History
- Published Online: 13 MAR 2003
- Published Print: 1 NOV 2002
ISBN Information
Print ISBN: 9780471159858
Online ISBN: 9780471267294
- Summary
- Chapter
Keywords:
- linear rates;
- hyperbolic kinetics;
- S/Km range;
- selectivity constant;
- substrate inhibition
Summary
The characterization of enzyme action requires the accurate and careful assessment of kinetic constants, most often as Vmax and Km. In evaluating original experimental data, or those reported in the literature, several conditions must be met to ensure proper kinetic characterization. In this chapter, simple kinetic principles are applied to illustrate and guide the evaluation and interpretation of enzyme kinetic behavior. In this context, examples will be used to illustrate some common shortcomings and flaws in the routine reporting of enzyme kinetic properties.