Chapter 15. Use of Enzyme Kinetic Data in the Study of Structure–Function Relationships of Proteins
- Alejandro G. Marangoni
Published Online: 13 MAR 2003
DOI: 10.1002/0471267295.ch15
Copyright © 2003 John Wiley & Sons, Inc.
Book Title
Enzyme Kinetics: A Modern Approach
Additional Information
How to Cite
Tanaka, T. and Yada, R. Y. (2003) Use of Enzyme Kinetic Data in the Study of Structure–Function Relationships of Proteins, in Enzyme Kinetics: A Modern Approach (ed A. G. Marangoni), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471267295.ch15
Editor Information
Department of Food Science, University of Guelph, Canada
Publication History
- Published Online: 13 MAR 2003
- Published Print: 1 NOV 2002
ISBN Information
Print ISBN: 9780471159858
Online ISBN: 9780471267294
- Summary
- Chapter
Keywords:
- structure–function;
- molecular biology;
- enzymes;
- kinetic parameters;
- aspartic proteinases
Summary
The ability to change specific residues/regions of proteins through the use of molecular biology techniques, e.g., site-directed mutagenesis, has allowed for rapid and greater advances in understanding the structure–function relationships in proteins. Integral to these studies is the analysis of enzyme kinetic data. The following chapter will examine how enzyme kinetic data, by posing various questions, can be used in protein structure–functions studies where molecular biology techniques have been used.