Chapter 4. Reversible Enzyme Inhibition
- Alejandro G. Marangoni
Published Online: 13 MAR 2003
DOI: 10.1002/0471267295.ch4
Copyright © 2003 John Wiley & Sons, Inc.
Book Title
Enzyme Kinetics: A Modern Approach
Additional Information
How to Cite
Marangoni, A. G. (2003) Reversible Enzyme Inhibition, in Enzyme Kinetics: A Modern Approach (ed A. G. Marangoni), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471267295.ch4
Editor Information
Department of Food Science, University of Guelph, Canada
Publication History
- Published Online: 13 MAR 2003
- Published Print: 1 NOV 2002
ISBN Information
Print ISBN: 9780471159858
Online ISBN: 9780471267294
- Summary
- Chapter
Keywords:
- reversible inhibition;
- Ki;
- competitive;
- uncompetitive;
- non-competitive;
- linear mixed-type
Summary
This chapter reviews the four basic types of reversible enzyme inhibition, namely competitive, uncompetitive, non-competitive and linear mixed-type. Equilibrium kinetic models are derived from first principles. Examples of the analysis of inhibition kinetic data for the determination of the inhibition constant, Ki, are presented. All analyses are exclusively carried out using non-linear regression techniques.