Chapter 4. Reversible Enzyme Inhibition

  1. Alejandro G. Marangoni
  1. Alejandro G. Marangoni

Published Online: 13 MAR 2003

DOI: 10.1002/0471267295.ch4

Enzyme Kinetics: A Modern Approach

Enzyme Kinetics: A Modern Approach

How to Cite

Marangoni, A. G. (2002) Reversible Enzyme Inhibition, in Enzyme Kinetics: A Modern Approach (ed A. G. Marangoni), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471267295.ch4

Editor Information

  1. Department of Food Science, University of Guelph, Canada

Author Information

  1. Department of Food Science, University of Guelph, Canada

Publication History

  1. Published Online: 13 MAR 2003
  2. Published Print: 1 NOV 2002

ISBN Information

Print ISBN: 9780471159858

Online ISBN: 9780471267294

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Keywords:

  • reversible inhibition;
  • Ki;
  • competitive;
  • uncompetitive;
  • non-competitive;
  • linear mixed-type

Summary

This chapter reviews the four basic types of reversible enzyme inhibition, namely competitive, uncompetitive, non-competitive and linear mixed-type. Equilibrium kinetic models are derived from first principles. Examples of the analysis of inhibition kinetic data for the determination of the inhibition constant, Ki, are presented. All analyses are exclusively carried out using non-linear regression techniques.