Chapter 6. pH Dependence of Enzyme-Catalyzed Reactions

  1. Alejandro G. Marangoni
  1. Alejandro G. Marangoni

Published Online: 13 MAR 2003

DOI: 10.1002/0471267295.ch6

Book Title

Enzyme Kinetics: A Modern Approach

Enzyme Kinetics: A Modern Approach

Additional Information

How to Cite

Marangoni, A. G. (2003) pH Dependence of Enzyme-Catalyzed Reactions, in Enzyme Kinetics: A Modern Approach (ed A. G. Marangoni), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471267295.ch6

Editor Information

  1. Department of Food Science, University of Guelph, Canada

Author Information

  1. Department of Food Science, University of Guelph, Canada

Publication History

  1. Published Online: 13 MAR 2003
  2. Published Print: 1 NOV 2002

ISBN Information

Print ISBN: 9780471159858

Online ISBN: 9780471267294

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Keywords:

  • pK;
  • pH;
  • functional groups;
  • pH-dependence

Summary

In this chapter, the theory of dependence of enzymatic reactions on pH is presented. A general model for the effect of pH on enzyme catalysis is derived from first principles. The effects of pH on catalytic parameters, Vmax and Km, are discussed in light of knowledge of the effects of acids and bases on reaction rates. A new graphical method for the determination of pK values of catalytically relevant residues within an enzyme is developed in this chapter as well, and compared to existing graphical methods.

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