Chapter 8. Multisite and Cooperative Enzymes
- Alejandro G. Marangoni
Published Online: 13 MAR 2003
DOI: 10.1002/0471267295.ch8
Copyright © 2003 John Wiley & Sons, Inc.
Book Title
Enzyme Kinetics: A Modern Approach
Additional Information
How to Cite
Marangoni, A. G. (2003) Multisite and Cooperative Enzymes, in Enzyme Kinetics: A Modern Approach (ed A. G. Marangoni), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471267295.ch8
Editor Information
Department of Food Science, University of Guelph, Canada
Publication History
- Published Online: 13 MAR 2003
- Published Print: 1 NOV 2002
ISBN Information
Print ISBN: 9780471159858
Online ISBN: 9780471267294
- Summary
- Chapter
Keywords:
- multisite;
- allosteric;
- cooperative;
- sequential interaction;
- concerted transition;
- Hill model
Summary
In this chapter, the kinetics of multisite and cooperative enzymes is discussed. The sequential interaction model is derived from first principles, consolidating existing models on the topic. Microscopic versus macroscopic dissociation constants are discussed. Reduction of the sequential interaction model to the Hill model is demonstrated. The concerted transition model is also developed from first principles and its assumptions discussed. The analysis of the activity of a two-substrate enzyme using both models is shown.