Chapter 8. Multisite and Cooperative Enzymes

  1. Alejandro G. Marangoni
  1. Alejandro G. Marangoni

Published Online: 13 MAR 2003

DOI: 10.1002/0471267295.ch8

Enzyme Kinetics: A Modern Approach

Enzyme Kinetics: A Modern Approach

How to Cite

Marangoni, A. G. (2002) Multisite and Cooperative Enzymes, in Enzyme Kinetics: A Modern Approach (ed A. G. Marangoni), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471267295.ch8

Editor Information

  1. Department of Food Science, University of Guelph, Canada

Author Information

  1. Department of Food Science, University of Guelph, Canada

Publication History

  1. Published Online: 13 MAR 2003
  2. Published Print: 1 NOV 2002

ISBN Information

Print ISBN: 9780471159858

Online ISBN: 9780471267294

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Keywords:

  • multisite;
  • allosteric;
  • cooperative;
  • sequential interaction;
  • concerted transition;
  • Hill model

Summary

In this chapter, the kinetics of multisite and cooperative enzymes is discussed. The sequential interaction model is derived from first principles, consolidating existing models on the topic. Microscopic versus macroscopic dissociation constants are discussed. Reduction of the sequential interaction model to the Hill model is demonstrated. The concerted transition model is also developed from first principles and its assumptions discussed. The analysis of the activity of a two-substrate enzyme using both models is shown.