Thermodynamics of the Binding of Ligands by Proteins

  1. Robert A. Alberty

Published Online: 28 JAN 2005

DOI: 10.1002/0471332607.ch7

Thermodynamics of Biochemical Reactions

Thermodynamics of Biochemical Reactions

How to Cite

Alberty, R. A. (2003) Thermodynamics of the Binding of Ligands by Proteins, in Thermodynamics of Biochemical Reactions, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471332607.ch7

Publication History

  1. Published Online: 28 JAN 2005
  2. Published Print: 13 FEB 2003

ISBN Information

Print ISBN: 9780471228516

Online ISBN: 9780471332602



  • binding;
  • oxygen;
  • hemoglobin tetramers;
  • Gibbs energy;
  • oxygen concentration;
  • tetramers;
  • dimers;
  • apparent equilibrium constants;
  • dissociation;
  • diprotic acid;
  • pH;
  • protein-ligand equilibria;
  • ligands binding;
  • fumarase


This chapter includes the following topics:

  • Binding of Oxygen by Hemoglobin Tetramers

  • Further Transformed Gibbs Energy at Specified Oxygen Concentration

  • Partial Dissociation of Tetramers into Dimers

  • Experimental Determination of Seven Apparent Equilibrium Constants

  • Dissociation of a Diprotic Acid

  • Effect of pH on Protein-Ligand Equilibria

  • Calculation of Standard Transformed Gibbs Energies of Formation of the Catalytic Site of Fumarase