Chapter 13. Voltage Clamp and Fluorometric Techniques for Studying Glutamate Transporter Function
- Michael W. Quick Ph.D.
Published Online: 19 MAR 2003
Copyright © 2002 by Wiley-Liss, Inc.
How to Cite
Tzingounis, A. V., Larsson, H. P. and Kavanaugh, M. P. (2002) Voltage Clamp and Fluorometric Techniques for Studying Glutamate Transporter Function, in Transmembrane Transporters (ed M. W. Quick), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471434043.ch13
Department of Neurobiology, University of Alabama at Birmingham School of Medicine, Birmingham, AL, USA
- Published Online: 19 MAR 2003
- Published Print: 23 AUG 2002
Book Series Editors:
- David R. Sibley
Series Editor Information
Molecular Neuropharmacology Section, Experimental Therapeutics Branch, NINDS, National Institutes of Health, Bethesda, Maryland, USA
Print ISBN: 9780471065135
Online ISBN: 9780471434047
- glutamate transporter;
- Xenopus oocytes;
- voltage clamp;
- concentration jump;
- patch recording
Glutamate transporters can influence the time course of synaptically released glutamate and maintain low ambient extracellular glutamate levels by accumulating it in the cell against a large gradient. Understanding these physiological processes requires a knowledge of the transporters' kinetics and structure-function relationships. In this chapter, we outline three methods - voltage clamp fluorometry, concentration jumps to outside-out patches, and computer modeling. These techniques provide a potentially powerful approach to study kinetics and the protein conformational changes during the transport cycle.