Chapter 13. Voltage Clamp and Fluorometric Techniques for Studying Glutamate Transporter Function

  1. Michael W. Quick Ph.D.
  1. Anastassios V. Tzingounis2,
  2. H. Peter Larsson1 and
  3. Michael P. Kavanaugh2

Published Online: 19 MAR 2003

DOI: 10.1002/0471434043.ch13

Transmembrane Transporters

Transmembrane Transporters

How to Cite

Tzingounis, A. V., Larsson, H. P. and Kavanaugh, M. P. (2002) Voltage Clamp and Fluorometric Techniques for Studying Glutamate Transporter Function, in Transmembrane Transporters (ed M. W. Quick), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471434043.ch13

Editor Information

  1. Department of Neurobiology, University of Alabama at Birmingham School of Medicine, Birmingham, AL, USA

Author Information

  1. 1

    Neurological Sciences Institute, Oregon Health and Science University, Portland, OR, USA

  2. 2

    Vollum Institute, Oregon Health and Sciences University, Portland, OR, USA

Publication History

  1. Published Online: 19 MAR 2003
  2. Published Print: 23 AUG 2002

Book Series:

  1. Receptor Biochemistry and Methodology

Book Series Editors:

  1. David R. Sibley

Series Editor Information

  1. Molecular Neuropharmacology Section, Experimental Therapeutics Branch, NINDS, National Institutes of Health, Bethesda, Maryland, USA

ISBN Information

Print ISBN: 9780471065135

Online ISBN: 9780471434047



  • glutamate transporter;
  • fluorescence;
  • Xenopus oocytes;
  • voltage clamp;
  • modeling;
  • concentration jump;
  • patch recording


Glutamate transporters can influence the time course of synaptically released glutamate and maintain low ambient extracellular glutamate levels by accumulating it in the cell against a large gradient. Understanding these physiological processes requires a knowledge of the transporters' kinetics and structure-function relationships. In this chapter, we outline three methods - voltage clamp fluorometry, concentration jumps to outside-out patches, and computer modeling. These techniques provide a potentially powerful approach to study kinetics and the protein conformational changes during the transport cycle.