Chapter 6. Nonviral Gene Transfer Allows Up- and Down-Expression of the Brain Serotonin Transporter with Functional Consequences

  1. Michael W. Quick Ph.D.
  1. Marie-Pascale Martres1,
  2. Véronique Fabre2,
  3. Michel Hamon2 and
  4. Barbara Demeneix3

Published Online: 19 MAR 2003

DOI: 10.1002/0471434043.ch6

Transmembrane Transporters

Transmembrane Transporters

How to Cite

Martres, M.-P., Fabre, V., Hamon, M. and Demeneix, B. (2002) Nonviral Gene Transfer Allows Up- and Down-Expression of the Brain Serotonin Transporter with Functional Consequences, in Transmembrane Transporters (ed M. W. Quick), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471434043.ch6

Editor Information

  1. Department of Neurobiology, University of Alabama at Birmingham School of Medicine, Birmingham, AL, USA

Author Information

  1. 1

    INSERM, Faculté de Médecine, Créteil, France

  2. 2

    INSERM, Faculte de Medecine, Paris, France

  3. 3

    CNRS UMR, Museum National d'Hisoire Naturelle, Paris, France

Publication History

  1. Published Online: 19 MAR 2003
  2. Published Print: 23 AUG 2002

Book Series:

  1. Receptor Biochemistry and Methodology

Book Series Editors:

  1. David R. Sibley

Series Editor Information

  1. Molecular Neuropharmacology Section, Experimental Therapeutics Branch, NINDS, National Institutes of Health, Bethesda, Maryland, USA

ISBN Information

Print ISBN: 9780471065135

Online ISBN: 9780471434047

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Keywords:

  • brain serotonin transporter;
  • plasmid DNA transfer;
  • polyethylenimine;
  • non mitotic neurons;
  • 5-HT turn-over;
  • 5-HT1A autoreceptors;
  • sleep-wakefulness rhythms;
  • antidepressants

Summary

We described a non-viral gene transfer in the adult rat brain, using the cationic polymer, polyethylenimine. Micro-injection of sense- or antisense coding sequence of the serotonin transporter (5-HTT) allows its up- and down-expression at the level of serotonin (5-HT) cell bodies and in various projection areas. These regulations of 5-HTT density induced variations in the 5-HT turn-over as well as in the coupling of the 5-HT1A autoreceptors and in sleep-wakefulness rhythms. The data obtained were compared with those elicited by antidepressants or 5-HT releasers. The advantages of this non-viral DNA transfer as compared to other technics are discussed.