6. Sample Preparation in DNA Analysis

  1. Somenath Mitra
  1. Satish Parimoo1 and
  2. Bhama Parimoo2

Published Online: 29 OCT 2003

DOI: 10.1002/0471457817.ch6

Sample Preparation Techniques in Analytical Chemistry, Volume 162

Sample Preparation Techniques in Analytical Chemistry, Volume 162

How to Cite

Parimoo, S. and Parimoo, B. (2003) Sample Preparation in DNA Analysis, in Sample Preparation Techniques in Analytical Chemistry, Volume 162 (ed S. Mitra), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471457817.ch6

Editor Information

  1. Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, NJ 07102, USA

Author Information

  1. 1

    Aderans Research Institute, Inc., 3701 Market Street, Philadelphia, PA 19104, USA

  2. 2

    Department of Pharmaceutical Chemistry, Rutgers University College of Pharmacy, Piscataway, NJ 08854, USA

Publication History

  1. Published Online: 29 OCT 2003
  2. Published Print: 19 SEP 2003

Book Series:

  1. Chemical Analysis

Book Series Editors:

  1. J. D. Winefordner

ISBN Information

Print ISBN: 9780471328452

Online ISBN: 9780471457817

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Keywords:

  • DNA;
  • phenolic extraction;
  • DNA purification;
  • DNA isolation;
  • plasmid DNA;
  • genomic DNA;
  • DNA from bacteria;
  • DNA from mammalian tissues;
  • polymerase chain reaction;
  • PCR;
  • DNA storage

Summary

This chapter describes sample preparation in DNA analysis. Structure of DNA and its physical/chemicals properties are discussed briefly. Phenolic extraction and the removal of contamination from DNA are described in significant details. Methods for the isolation of plasmid DNA, genomic DNA, DNA from mammalian tissues, yeast, bacteria, and the extraction of high molecular weight DNA are presented. Polymerase chain reaction (PCR) for DNA amplification has been discussed with examples of small real-world samples. Methods for the assessment of quality and DNA quantity are also discussed, along with precautions in DNA handling and storage.