7. Sample Preparation in RNA Analysis

  1. Somenath Mitra
  1. Bhama Parimoo1 and
  2. Satish Parimoo2

Published Online: 29 OCT 2003

DOI: 10.1002/0471457817.ch7

Sample Preparation Techniques in Analytical Chemistry, Volume 162

Sample Preparation Techniques in Analytical Chemistry, Volume 162

How to Cite

Parimoo, B. and Parimoo, S. (2003) Sample Preparation in RNA Analysis, in Sample Preparation Techniques in Analytical Chemistry, Volume 162 (ed S. Mitra), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471457817.ch7

Editor Information

  1. Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, NJ 07102, USA

Author Information

  1. 1

    Department of Pharmaceutical Chemistry, Rutgers University College of Pharmacy, Piscataway, NJ 08854, USA

  2. 2

    Aderans Research Institute, Inc., 3701 Market Street, Philadelphia, PA 19104, USA

Publication History

  1. Published Online: 29 OCT 2003
  2. Published Print: 19 SEP 2003

Book Series:

  1. Chemical Analysis

Book Series Editors:

  1. J. D. Winefordner

ISBN Information

Print ISBN: 9780471328452

Online ISBN: 9780471457817

SEARCH

Keywords:

  • RNA;
  • phenolic extraction;
  • guanidium salt method;
  • RNA purification, and removal of DNA during RNA isolation;
  • RNA storage

Summary

Structure of RNA, its physical/chemicals properties and basic considerations in RNA analysis begin the discussion in this chapter. Methods of RNA extraction, particularly phenolic extraction and guanidium salt methods are described along with methods of purification, such as the removal of DNA from RNA. Examples of RNA isolation from nuclear and cytoplasmic cellular fraction are used as examples. RNA fractionation by chromatography and the RNA isolation from small number of cells are presented. Finally the assessment of quality, and quantitation of RNA are described.