8. Techniques for the Extraction, Isolation, and Purification of Nucleic Acids

  1. Somenath Mitra
  1. Mahesh Karwa and
  2. Somenath Mitra

Published Online: 29 OCT 2003

DOI: 10.1002/0471457817.ch8

Sample Preparation Techniques in Analytical Chemistry, Volume 162

Sample Preparation Techniques in Analytical Chemistry, Volume 162

How to Cite

Karwa, M. and Mitra, S. (2003) Techniques for the Extraction, Isolation, and Purification of Nucleic Acids, in Sample Preparation Techniques in Analytical Chemistry, Volume 162 (ed S. Mitra), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471457817.ch8

Editor Information

  1. Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, NJ 07102, USA

Author Information

  1. Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, NJ 07102, USA

Publication History

  1. Published Online: 29 OCT 2003
  2. Published Print: 19 SEP 2003

Book Series:

  1. Chemical Analysis

Book Series Editors:

  1. J. D. Winefordner

ISBN Information

Print ISBN: 9780471328452

Online ISBN: 9780471457817

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Keywords:

  • cell lysis;
  • membrane filtration;
  • size-exclusion chromatography;
  • anion-exchange chromatography;
  • solid-phase extraction;
  • solid-phase reversible immobilization;
  • affinity purification;
  • triple helix affinity capture;
  • high-throughput DNA purification systems;
  • electrophoresis;
  • gel electrophoresis;
  • capillary electrophoresis (CE);
  • capillary gel electrophoresis (CGE);
  • μ-TAS;
  • microfabricated device

Summary

This chapter provides and overview of different techniques for the extraction, isolation and purification of nucleic acids. Mechanical and non-mechanical means of cell lysis are described. This is followed by discussions on the methods of nucleic acids purification from cell debris and other interfering components. The techniques covered are membrane filtration, chromatography, solid phase extraction, affinity purification, and electrophoresis. A brief discussion on automated high throughput DNA purification systems is also included. Finally, a few examples of sample preparation on microchips, as they lead to the development of μ-TAS (Micro-total analytical systems) are presented.