Chapter 8. Manufacturing Protein

  1. Stephen R. Bolsover1,
  2. Jeremy S. Hyams2,
  3. Elizabeth A. Shephard3,
  4. Hugh A. White3,
  5. Claudia G. Wiedemann1

Published Online: 27 JAN 2004

DOI: 10.1002/047146158X.ch8

Book Title

Cell Biology: A Short Course, Second Edition

Cell Biology: A Short Course, Second Edition

Additional Information

How to Cite

Bolsover, S. R., Hyams, J. S., Shephard, E. A., White, H. A. and Wiedemann, C. G. (2004) Manufacturing Protein, in Cell Biology: A Short Course, Second Edition, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/047146158X.ch8

Author Information

  1. 1

    Department of Physiology, University College, London, UK

  2. 2

    Department of Biology, University College, London, UK

  3. 3

    Department of Biochemistry and Molecular Biology, University College, London, UK

Publication History

  1. Published Online: 27 JAN 2004
  2. Published Print: 14 NOV 2003

ISBN Information

Print ISBN: 9780471263937

Online ISBN: 9780471461586

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Keywords:

  • transfer RNA;
  • ribosome;
  • anticodon;
  • wobble;
  • polyribosome;
  • antibiotic;
  • SDS-PAGE;
  • electrophoresis

Summary

In this chapter we describe how proteins are made at the prokaryotic and eukaryotic ribosome, tRNAs specific for particular codons carry amino acids which are joined to the growing polypeptide. We explain initiation and termination of protein synthesis and the mechanism of antibiotics such as puromycin. SDS-PAGE and two-dimensional gel electrophoresis allow separation of different proteins from a sample, while western blotting and proteomics allow their identification.

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