2. The Basic Toolbox–Acquiring Functional Genomic Data

  1. Christopher A. Cullis

Published Online: 26 JAN 2004

DOI: 10.1002/0471488577.ch2

Plant Genomics and Proteomics

Plant Genomics and Proteomics

How to Cite

Cullis, C. A. (2003) The Basic Toolbox–Acquiring Functional Genomic Data, in Plant Genomics and Proteomics, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471488577.ch2

Publication History

  1. Published Online: 26 JAN 2004
  2. Published Print: 24 DEC 2003

ISBN Information

Print ISBN: 9780471373148

Online ISBN: 9780471488576



  • genome libraries;
  • transcription profiling;
  • BACs;
  • ESTs;
  • MPSS;
  • SAGE;
  • sequence analysis


Strategies for analyzing the genome include genomic sequencing, cDNA sequencing, expression profiling and protein characterization. Genome sequencing strategies can be based on BAC-based physical maps, shotgun sequencing or a combination of both. Expression can be characterized through microarrays, ESTs, SAGE and MPSS profiling. Protein profiling and sequencing can be done using mass spectrometry. All the data from these high throughput technologies is housed in relational databases for analysis.