Chapter 2. The Basic Toolbox–Acquiring Functional Genomic Data
Published Online: 26 JAN 2004
DOI: 10.1002/0471488577.ch2
Copyright © 2004 John Wiley & Sons, Inc.
Book Title
Plant Genomics and Proteomics
Additional Information
How to Cite
Cullis, C. A. (2004) The Basic Toolbox–Acquiring Functional Genomic Data, in Plant Genomics and Proteomics, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471488577.ch2
Publication History
- Published Online: 26 JAN 2004
- Published Print: 24 DEC 2003
ISBN Information
Print ISBN: 9780471373148
Online ISBN: 9780471488576
- Summary
- Chapter
Keywords:
- genome libraries;
- transcription profiling;
- BACs;
- ESTs;
- MPSS;
- SAGE;
- sequence analysis
Summary
Strategies for analyzing the genome include genomic sequencing, cDNA sequencing, expression profiling and protein characterization. Genome sequencing strategies can be based on BAC-based physical maps, shotgun sequencing or a combination of both. Expression can be characterized through microarrays, ESTs, SAGE and MPSS profiling. Protein profiling and sequencing can be done using mass spectrometry. All the data from these high throughput technologies is housed in relational databases for analysis.