Chapter 3. Sequencing Strategies
Published Online: 26 JAN 2004
DOI: 10.1002/0471488577.ch3
Copyright © 2004 John Wiley & Sons, Inc.
Book Title
Plant Genomics and Proteomics
Additional Information
How to Cite
Cullis, C. A. (2004) Sequencing Strategies, in Plant Genomics and Proteomics, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471488577.ch3
Publication History
- Published Online: 26 JAN 2004
- Published Print: 24 DEC 2003
ISBN Information
Print ISBN: 9780471373148
Online ISBN: 9780471488576
- Summary
- Chapter
Keywords:
- complete genome sequence;
- physical and genetic maps;
- methyl filtration;
- reassociation kinetics;
- BAC-end sequencing
Summary
Genomic sequence data can be obtained from whole genome sequencing projects or from fragments enriched by methyl filtration reassociation methods. Whole genomes have been sequenced using the minimal tiling path (Arabidopsis) and shotgun (Arabidopsis and rice) strategies. The sequence data have to be assembled into contigs and these placed on the genetic and physical maps for subsequent use in comparative genomics.