4. Gene Discovery

  1. Christopher A. Cullis

Published Online: 26 JAN 2004

DOI: 10.1002/0471488577.ch4

Plant Genomics and Proteomics

Plant Genomics and Proteomics

How to Cite

Cullis, C. A. (2003) Gene Discovery, in Plant Genomics and Proteomics, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471488577.ch4

Publication History

  1. Published Online: 26 JAN 2004
  2. Published Print: 24 DEC 2003

ISBN Information

Print ISBN: 9780471373148

Online ISBN: 9780471488576

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Keywords:

  • ESTs;
  • cDNAs;
  • insertional mutagenesis;
  • TILLING;
  • RNAi

Summary

Genes need to be identified within the genome. This can be through the characterization of cDNAs and the annotation of the genome using gene finding programs. Alternatively, functional assays involving activation tagging, insertional mutagenesis with transposons or T-DNA, and chemical mutagenesis. RNAi that induces epigenetic suppression of homologous genes (gene silencing) is particularly useful for identifying gene families with redundant/overlapping functions.