Chapter 6. Functional Genomics
Published Online: 26 JAN 2004
DOI: 10.1002/0471488577.ch6
Copyright © 2004 John Wiley & Sons, Inc.
Book Title
Plant Genomics and Proteomics
Additional Information
How to Cite
Cullis, C. A. (2004) Functional Genomics, in Plant Genomics and Proteomics, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471488577.ch6
Publication History
- Published Online: 26 JAN 2004
- Published Print: 24 DEC 2003
ISBN Information
Print ISBN: 9780471373148
Online ISBN: 9780471488576
- Summary
- Chapter
Keywords:
- transcription profiling;
- microarrays;
- SAGE;
- MPSS;
- yeast two-hybrids;
- affinity tagging
Summary
The genomic information has to be processed from the DNA instructions to functional molecules, be they RNAs, proteins or metabolites. Differential transcription can be characterized using microarrays, SAGE and MPSS. Proteins are analyzed using two-dimensional gel electrophoresis followed by mass spectrometry. The interactions between proteins can be identified via the yeast two-hybrid system or by in vivo affinity tagging. Metabolomics, the characterization of all the metabolites is still in its infancy.