Chapter 10. Applications of Zone Electrophoresis
Published Online: 26 FEB 2004
DOI: 10.1002/0471623628.ch10
Copyright © 2004 John Wiley & Sons, Inc.
Book Title
Bioanalytical Chemistry
Additional Information
How to Cite
Mikkelsen, S. R. and Cortón, E. (2004) Applications of Zone Electrophoresis, in Bioanalytical Chemistry, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471623628.ch10
Publication History
- Published Online: 26 FEB 2004
- Published Print: 20 FEB 2004
ISBN Information
Print ISBN: 9780471544470
Online ISBN: 9780471623625
- Summary
- Chapter
Keywords:
- zone electrophoresis;
- molecular weight;
- net charge;
- subunit composition;
- isoenzymes;
- DNA fingerprinting;
- DNA sequencing;
- immunoelectrophoresis
Summary
Applications of zone electrophoresis, including methods for the determination of protein net charge and molecular weight using PAGE and the Ferguson method, determination of protein subunit composition and subunit molecular weight, molecular weight of DNA by agarose gel electrophoresis, identification of isoenzymes, diagnosis of genetic (inherited) disease, DNA fingerprinting and restriction fragment length polymorphism, DNA sequencing with the Maxam–Gilbert method, immunoelectrophoresis.