Chapter 11. Isoelectric Focusing

  1. Susan R. Mikkelsen1,
  2. Eduardo Cortón2

Published Online: 26 FEB 2004

DOI: 10.1002/0471623628.ch11

Book Title

Bioanalytical Chemistry

Bioanalytical Chemistry

Additional Information

How to Cite

Mikkelsen, S. R. and Cortón, E. (2004) Isoelectric Focusing, in Bioanalytical Chemistry, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471623628.ch11

Author Information

  1. 1

    University of Waterloo, Ontario, Canada

  2. 2

    University of Buenos Aires, Argentina

Publication History

  1. Published Online: 26 FEB 2004
  2. Published Print: 20 FEB 2004

ISBN Information

Print ISBN: 9780471544470

Online ISBN: 9780471623625

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CHAPTER TOOLS

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Keywords:

  • isoelectric focussing;
  • pH gradients;
  • carrier ampholytes;
  • immobilized pH gradients;
  • two-dimensional electrophoresis

Summary

Principles and applications of isoelectric focussing, including principles of pH gradients, generating and stabilizing pH gradients, modern IEF with carrier ampholytes, immobilized pH gradients (IPGs), two-dimensional electrophoresis.

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