Chapter 13. Centrifugation Methods

Summary

Principles and applications of centrifugation methods, including sedimentation and relative centrifugal g force, centrifugal forces in different rotor types, swinging-bucket rotors, fixed-angle rotors, vertical rotors, clearing factor (k), density gradients, materials used to generate density gradients, pre-formed and self-generated density gradients, redistribution of gradients in fixed-angle and vertical rotors, differential centrifugation, rate-zonal centrifugation, isopycnic centrifugation, harvesting samples, analytical ultracentrifugation, instrumentation, sedimentation velocity analysis, sedimentation equilibrium analysis, analytical ultracentrifugation for quaternary structure elucidation, isolation of retroviruses by self-generated gradients, isolation of lipoproteins from human plasma.