Chapter 14. Chromatography of Biomolecules

  1. Susan R. Mikkelsen1,
  2. Eduardo Cortón2

Published Online: 26 FEB 2004

DOI: 10.1002/0471623628.ch14

Book Title

Bioanalytical Chemistry

Bioanalytical Chemistry

Additional Information

How to Cite

Mikkelsen, S. R. and Cortón, E. (2004) Chromatography of Biomolecules, in Bioanalytical Chemistry, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471623628.ch14

Author Information

  1. 1

    University of Waterloo, Ontario, Canada

  2. 2

    University of Buenos Aires, Argentina

Publication History

  1. Published Online: 26 FEB 2004
  2. Published Print: 20 FEB 2004

ISBN Information

Print ISBN: 9780471544470

Online ISBN: 9780471623625

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CHAPTER TOOLS

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Keywords:

  • chromatography;
  • units;
  • definitions;
  • plate theory;
  • rate theory;
  • size exclusion;
  • gel filtration;
  • affinity chromatography;
  • elution methods;
  • association constants;
  • ion-exchange chromatography

Summary

Chromatography of macromolecular biomolecules, including units and definitions, plate theory, rate theory, size exclusion (gel filtration), gel matrices for size exclusion chromatography, affinity chromatography, immobilization of affinity ligands, elution methods, determination of association constants by high performance affinity chromatography, ion-exchange chromatography, retention model for ion-exchange chromatography of polyelectrolytes.

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