Chapter 2. Enzymes

  1. Susan R. Mikkelsen1,
  2. Eduardo Cortón2

Published Online: 26 FEB 2004

DOI: 10.1002/0471623628.ch2

Book Title

Bioanalytical Chemistry

Bioanalytical Chemistry

Additional Information

How to Cite

Mikkelsen, S. R. and Cortón, E. (2004) Enzymes, in Bioanalytical Chemistry, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471623628.ch2

Author Information

  1. 1

    University of Waterloo, Ontario, Canada

  2. 2

    University of Buenos Aires, Argentina

Publication History

  1. Published Online: 26 FEB 2004
  2. Published Print: 20 FEB 2004

ISBN Information

Print ISBN: 9780471544470

Online ISBN: 9780471623625

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Keywords:

  • enzymes;
  • enzyme kinetics;
  • applications;
  • Km values;
  • enzyme activators;
  • enzyme inhibitors;
  • enzyme units and concentrations

Summary

Introduction to basic enzyme-related concepts, including enzyme nomenclature, applications of enzymes in bioanalytical chemistry, enzyme kinetics, simple one-substrate enzyme kinetics and their determination using different experimental methods, one-substrate/two-product enzyme kinetics, two-substrate enzyme kinetics, examples of enzyme-catalyzed reactions and their treatment, enzyme activators, enzyme inhibitors, competitive, noncompetitive and uncompetitive inhibition, enzyme units and concentrations.

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