Chapter 3. Quantitation of Enzymes and Their Substrates
Published Online: 26 FEB 2004
DOI: 10.1002/0471623628.ch3
Copyright © 2004 John Wiley & Sons, Inc.
Book Title
Bioanalytical Chemistry
Additional Information
How to Cite
Mikkelsen, S. R. and Cortón, E. (2004) Quantitation of Enzymes and Their Substrates, in Bioanalytical Chemistry, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471623628.ch3
Publication History
- Published Online: 26 FEB 2004
- Published Print: 20 FEB 2004
ISBN Information
Print ISBN: 9780471544470
Online ISBN: 9780471623625
- Summary
- Chapter
Keywords:
- enzymes;
- substrate depletion;
- product accumulation;
- coupled measurements;
- optical detection;
- electrochemical detection;
- ultra-high-throughput assays;
- practical considerations
Summary
Methods for the quantitation of enzymes and their substrates, including substrate depletion and product accumulation measurements, direct and coupled measurements, indicator reactions, classification of methods to measure enzymatic activity, including optical (absorbance, fluorescence, luminescence), electrochemical (amperometry, potentiometry and conductimetry), and other methods, ultra-high-throughput assays, practical considerations for enzymatic assays.