Chapter 4. Immobilized Enzymes
Published Online: 26 FEB 2004
DOI: 10.1002/0471623628.ch4
Copyright © 2004 John Wiley & Sons, Inc.
Book Title
Bioanalytical Chemistry
Additional Information
How to Cite
Mikkelsen, S. R. and Cortón, E. (2004) Immobilized Enzymes, in Bioanalytical Chemistry, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471623628.ch4
Publication History
- Published Online: 26 FEB 2004
- Published Print: 20 FEB 2004
ISBN Information
Print ISBN: 9780471544470
Online ISBN: 9780471623625
- Summary
- Chapter
Keywords:
- immobilized enzymes;
- immobilization methods;
- immobilization supports;
- functional groups;
- crosslinking;
- bifunctional reagents;
- properties of immobilized enzymes;
- immobilized enzyme reactors;
- theoretical treatment
Summary
Methods for enzyme immobilization, their characterization and theoretical considerations of practical importance, including nonpolymerizing covalent immobilization methods (controlled-pore glass, polysaccharides, polyacrylamide, acidic supports, anhydride groups and thiol groups), crosslinking with bifunctional reagents, adsorption, entrapment, microencapsulation, properties of immobilized enzymes, immobilized enzyme reactors, theoretical treatment of packed-bed enzyme reactors.