Chapter 8. Directed Evolution for the Design of Macromolecular Bioassay Reagents

  1. Susan R. Mikkelsen1,
  2. Eduardo Cortón2

Published Online: 26 FEB 2004

DOI: 10.1002/0471623628.ch8

Book Title

Bioanalytical Chemistry

Bioanalytical Chemistry

Additional Information

How to Cite

Mikkelsen, S. R. and Cortón, E. (2004) Directed Evolution for the Design of Macromolecular Bioassay Reagents, in Bioanalytical Chemistry, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471623628.ch8

Author Information

  1. 1

    University of Waterloo, Ontario, Canada

  2. 2

    University of Buenos Aires, Argentina

Publication History

  1. Published Online: 26 FEB 2004
  2. Published Print: 20 FEB 2004

ISBN Information

Print ISBN: 9780471544470

Online ISBN: 9780471623625

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Keywords:

  • directed evolution;
  • rational design;
  • error-prone PCR;
  • DNA shuffling;
  • cell surface display;
  • phage display;
  • ribosome display;
  • mRNA–peptide fusion;
  • microcompartmentalization;
  • identification and selection

Summary

Directed evolution methods and their application to the design of macromolecular bioassay reagents, including rational design and directed evolution, generation of genetic diversity, polymerase chain reaction and error-prone PCR, DNA shuffling, methods for linking genotype to phenotype, cell expression and cell surface display, phage display, ribosome display, mRNA–peptide fusion, microcompartmentalization, identification and selection of successful variants, directed evolution of galactose oxidase.

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