Chapter 2. Overview of “Traditional” Experimental Arsenal to Study Biomolecular Structure and Dynamics

  1. Igor A. Kaltashov and
  2. Stephen J. Eyles

Published Online: 27 JAN 2005

DOI: 10.1002/0471705179.ch2

Mass Spectrometry in Biophysics: Conformation and Dynamics of Biomolecules

Mass Spectrometry in Biophysics: Conformation and Dynamics of Biomolecules

How to Cite

Kaltashov, I. A. and Eyles, S. J. (2005) Overview of “Traditional” Experimental Arsenal to Study Biomolecular Structure and Dynamics, in Mass Spectrometry in Biophysics: Conformation and Dynamics of Biomolecules, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471705179.ch2

Author Information

  1. University of Massachusetts at Amherst, USA

Publication History

  1. Published Online: 27 JAN 2005
  2. Published Print: 6 APR 2005

Book Series:

  1. Wiley-Interscience Series in Mass Spectrometry

Book Series Editors:

  1. Dominic M. Desiderio2 and
  2. Nico M. M. Nibbering3

Series Editor Information

  1. 2

    Departments of Neurology and Biochemistry, University of Tennessee Health Science Center, USA

  2. 3

    Vrije Universiteit Amsterdam, The Netherlands

ISBN Information

Print ISBN: 9780471456025

Online ISBN: 9780471705178

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Keywords:

  • X-ray cystallography;
  • X-ray scattering;
  • light scattering;
  • NMR spectroscopy;
  • circular dichroism;
  • fluorescence;
  • infra-red spectroscopy;
  • calorimetry;
  • surface plasmon resonance

Summary

Biophysicists traditionally use a battery of experimental techniques, which have not until relatively recently included mass spectrometry. The purpose of this chapter is to present an overview of the alternative methodologies that have been routinely employed, explaining the details which can be gleaned from each technique. This will be used as a jump point in future chapters to demonstrate the complementary nature of mass spectrometric methodologies in the biophysical arena.