Flow Sorting

  1. Howard M. Shapiro

Published Online: 28 JAN 2005

DOI: 10.1002/0471722731.ch6

Practical Flow Cytometry

Practical Flow Cytometry

How to Cite

Shapiro, H. M. (2003) Flow Sorting, in Practical Flow Cytometry, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471722731.ch6

Publication History

  1. Published Online: 28 JAN 2005
  2. Published Print: 30 JUN 2003

ISBN Information

Print ISBN: 9780471411253

Online ISBN: 9780471722731

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Keywords:

  • fluidic switching;
  • droplet sorting;
  • shift register;
  • clock signal

Summary

This chapter includes the following topics:

  • Sort Control (Decision) Logic

  • Preselected Count Circuits and Single Cell Sorting

  • Droplet Sorting, High-Speed and Low

  • Fluidic Switching Cell Sorters

  • Cell Manipulation by Optical Trapping

  • Cell Damage Selection (“Cell Zapping”)

  • Measures of Cell Sorter Performance: Purity, Recovery (Yield), and Efficiency

  • Other Considerations

  • Biohazard Control and Biosafety in Flow Cytometers and Sorters

  • Conclusions