Parameters and Probes

  1. Howard M. Shapiro

Published Online: 28 JAN 2005

DOI: 10.1002/0471722731.ch7

Practical Flow Cytometry

Practical Flow Cytometry

How to Cite

Shapiro, H. M. (2003) Parameters and Probes, in Practical Flow Cytometry, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471722731.ch7

Publication History

  1. Published Online: 28 JAN 2005
  2. Published Print: 30 JUN 2003

ISBN Information

Print ISBN: 9780471411253

Online ISBN: 9780471722731



  • Coulter principle;
  • lymphocyte gating;
  • back-gating;
  • nuclear morphology


This chapter includes the following topics:

  • Physical Parameters

  • Intrinsic Cellular Parameters

  • Probes, Labels, and [Not] Protocols for Extrinsic Parameter Measurements

  • Nucleic Acid Dyes and Their Uses

  • Fluorescent Labels and Protein Dyes

  • Improving Signals from Labels: Amplification and Other Techniques

  • Measuring Cell Surface and Intracellular Antigens

  • Nucleic Acid Sequence Detection

  • Probes for Various Cell Constituents

  • Time as a Parameter: Kinetic Measurements

  • Labeled Ligand Binding

  • Functional Parameters I

  • Functional Probes II: Indicators of Cell Activation

  • Reporter Genes