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Microscopy and Spectroscopy, Near-Field

  1. Miodrag Micic

Published Online: 14 APR 2006

DOI: 10.1002/0471732877.emd294

Encyclopedia of Medical Devices and Instrumentation

Encyclopedia of Medical Devices and Instrumentation

How to Cite

Micic, M. 2006. Microscopy and Spectroscopy, Near-Field. Encyclopedia of Medical Devices and Instrumentation. .

Author Information

  1. MP Biomedicals LLC, Irvine, California

Publication History

  1. Published Online: 14 APR 2006

Abstract

This article reviews the principles and applications of optical imaging beyond the diffraction limit using near-field optics. Such a technique allows optical imaging and spectroscopy with lateral resolution up to 20 nm, and is promising to revolutionize research in life science disciplines, with a possible plethora of practical biomedical applications, especially in the field of in vitro diagnostics. The article provides an introduction to the near-field optics as applied in microscopy, and the fundamental concepts behind the two basic types of near-field scanning optical microscopes: aperture-based and apertureless, along with examples of life science and biomedical applications.

Keywords:

  • microscopy;
  • optics;
  • near-field;
  • scanning probe;
  • NSOM;
  • SNOM;
  • raman spectroscopy;
  • fluorescence spectroscopy;
  • optical fiber