Microscopy and Spectroscopy, Near-Field
Published Online: 14 APR 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Encyclopedia of Medical Devices and Instrumentation
How to Cite
Micic, M. 2006. Microscopy and Spectroscopy, Near-Field. Encyclopedia of Medical Devices and Instrumentation. .
- Published Online: 14 APR 2006
This article reviews the principles and applications of optical imaging beyond the diffraction limit using near-field optics. Such a technique allows optical imaging and spectroscopy with lateral resolution up to 20 nm, and is promising to revolutionize research in life science disciplines, with a possible plethora of practical biomedical applications, especially in the field of in vitro diagnostics. The article provides an introduction to the near-field optics as applied in microscopy, and the fundamental concepts behind the two basic types of near-field scanning optical microscopes: aperture-based and apertureless, along with examples of life science and biomedical applications.
- scanning probe;
- raman spectroscopy;
- fluorescence spectroscopy;
- optical fiber