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Cellular Imaging

  1. Ammasi Periasamy

Published Online: 14 APR 2006

DOI: 10.1002/0471732877.emd327

Encyclopedia of Medical Devices and Instrumentation

Encyclopedia of Medical Devices and Instrumentation

How to Cite

Periasamy, A. 2006. Cellular Imaging. Encyclopedia of Medical Devices and Instrumentation. .

Author Information

  1. University of Virginia, Charlottesville, Virginia

Publication History

  1. Published Online: 14 APR 2006


Microscopy becomes an essential tool for cellular imaging. The development of new fluorescent markers, such as green fluorescent proteins, generated great interest to measure cellular signals qualitatively and quantitatively using advanced fluorescence microscope techniques including wide-field, confocal, multiphoton, lifetime, Förster resonance energy-transfer (FRET), and correlation spectroscopy. The application dictates the selection of microscope methodology to investigate the cellular dynamics. In this article, we explore selected applications such as localization of protein molecules using FRET microscopy and drug cellular uptake using multiphoton and lifetime imaging microscopy. Nonfluorescent techniques, such as Raman and coherent antistokes Raman spectroscopy (CARS), are also described for lipid imaging.


  • microscopy;
  • cellular imaging;
  • confocal;
  • FRET;
  • FLIM;
  • CARS;
  • raman spectroscopy;
  • multiphoton;
  • correlation spectroscopy;
  • drug uptake;
  • protein localization