Published Online: 14 APR 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Encyclopedia of Medical Devices and Instrumentation
How to Cite
Periasamy, A. 2006. Cellular Imaging. Encyclopedia of Medical Devices and Instrumentation. .
- Published Online: 14 APR 2006
Microscopy becomes an essential tool for cellular imaging. The development of new fluorescent markers, such as green fluorescent proteins, generated great interest to measure cellular signals qualitatively and quantitatively using advanced fluorescence microscope techniques including wide-field, confocal, multiphoton, lifetime, Förster resonance energy-transfer (FRET), and correlation spectroscopy. The application dictates the selection of microscope methodology to investigate the cellular dynamics. In this article, we explore selected applications such as localization of protein molecules using FRET microscopy and drug cellular uptake using multiphoton and lifetime imaging microscopy. Nonfluorescent techniques, such as Raman and coherent antistokes Raman spectroscopy (CARS), are also described for lipid imaging.
- cellular imaging;
- raman spectroscopy;
- correlation spectroscopy;
- drug uptake;
- protein localization