15. Tissue Engineering of the Liver

  1. Gordana Vunjak-Novakovic PhD2 and
  2. R. Ian Freshney PhD3
  1. Gregory H. Underhill,
  2. Jennifer Felix,
  3. Jared W. Allen,
  4. Valerie Liu Tsang,
  5. Salman R. Khetani and
  6. Sangeeta N. Bhatia

Published Online: 9 FEB 2006

DOI: 10.1002/0471741817.ch15

Culture of Cells for Tissue Engineering

Culture of Cells for Tissue Engineering

How to Cite

Underhill, G. H., Felix, J., Allen, J. W., Liu Tsang, V., Khetani, S. R. and Bhatia, S. N. (2005) Tissue Engineering of the Liver, in Culture of Cells for Tissue Engineering (eds G. Vunjak-Novakovic and R. I. Freshney), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471741817.ch15

Editor Information

  1. 2

    Department of Biomedical Engineering, Columbia University, 363G Engineering Terrace, Mail Code 8904, 1210 Amsterdam Avenue, New York, NY 10027, USA

  2. 3

    Centre for Oncology and Applied Pharmacology, University of Glasgow, Cancer Research UK Beatson Laboratories, Garscube Estate, Bearsden, Glasgow G61 1BD, Scotland, UK

Author Information

  1. Division of Health Sciences and Technology (Harvard-M.I.T.), Massachusetts Institute of Technology, 77 Massachusetts Avenue, E19–502D, Cambridge, Massachusetts, 02139, USA

Publication History

  1. Published Online: 9 FEB 2006
  2. Published Print: 11 APR 2005

Book Series:

  1. Culture of Specialized Cells

Book Series Editors:

  1. R. Ian Freshney PhD

Series Editor Information

  1. Centre for Oncology and Applied Pharmacology, University of Glasgow, Cancer Research UK Beatson Laboratories, Garscube Estate, Bearsden, Glasgow G61 1BD, Scotland, UK

ISBN Information

Print ISBN: 9780471629351

Online ISBN: 9780471741817

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Keywords:

  • primary hepatocyte stabilization;
  • micropatterned cell cultures;
  • stabilized hepatocyte culture systems

Summary

This chapter contains sections titled:

  • Background

  • Preparation of Reagents

  • Isolation of Primary Rat Hepatocytes

  • Assays of Hepatocyte Function

  • Micropatterned Cell Cultures

  • Bioreactor System for the Regulation of Hepatocyte Zonal Heterogeneity

  • Photopatterned Three-Dimensional Hydrogels Containing Living Cells

  • Discussion

  • Acknowledgments

  • Sources of Materials

  • References