5. Method Reproducibility and Spectral Library Assembly for Rapid Bacterial Characterization by Metastable Atom Bombardment Pyrolysis Mass Spectrometry

  1. Charles L. Wilkins3 and
  2. Jackson O. Lay Jr.4
  1. Jon G. Wilkes1,
  2. Gary Miertschin1,
  3. Todd Eschler1,
  4. Les Hosey1,
  5. Fatemeh Rafii2,
  6. Larry Rushing1,
  7. Dan A. Buzatu1 and
  8. Michel J. Bertrand1

Published Online: 3 JAN 2006

DOI: 10.1002/0471748641.ch5

Identification of Microorganisms by Mass Spectrometry, Volume 169

Identification of Microorganisms by Mass Spectrometry, Volume 169

How to Cite

Wilkes, J. G., Miertschin, G., Eschler, T., Hosey, L., Rafii, F., Rushing, L., Buzatu, D. A. and Bertrand, M. J. (2005) Method Reproducibility and Spectral Library Assembly for Rapid Bacterial Characterization by Metastable Atom Bombardment Pyrolysis Mass Spectrometry, in Identification of Microorganisms by Mass Spectrometry, Volume 169 (eds C. L. Wilkins and J. O. Lay), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/0471748641.ch5

Editor Information

  1. 3

    Department of Chemistry, University of Arkansas, Fayetteville, AR 72701, USA

  2. 4

    University of Arkansas, Fayetteville, AR 72701, USA

Author Information

  1. 1

    National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, USA

  2. 2

    Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, USA

Publication History

  1. Published Online: 3 JAN 2006
  2. Published Print: 14 OCT 2005

Book Series:

  1. Chemical Analysis

Book Series Editors:

  1. J. D. Winefordner

ISBN Information

Print ISBN: 9780471654421

Online ISBN: 9780471748649

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Keywords:

  • instrumental cell isolation;
  • autosampler and autoprobe;
  • PD-ANN algorithm

Summary

This chapter contains sections titled:

  • Introduction

  • Sample Preparation for Rapid, Reproducible Cell Culture

  • Analytical Instrumentation for Sensitive Detection and Spectral Reproducibility

  • Spectral Library Assembly

  • Pattern Recognition Methods for Objectively Classifying Bacteria

  • Conclusions

  • Acknowledgment

  • References