Human cytomegalovirus strains associated with congenital and perinatal infections †

  1. Daniel E. Trincado1,2,
  2. Gillian M. Scott1,
  3. Peter A. White1,2,
  4. Cheryl Hunt3,
  5. Lucy Rasmussen4,
  6. William D. Rawlinson1,2,*

Article first published online: 10 JUL 2000

DOI: 10.1002/1096-9071(200008)61:4<481::AID-JMV11>3.0.CO;2-H

Issue

Journal of Medical Virology

Journal of Medical Virology

Volume 61, Issue 4, pages 481–487, August 2000

Additional Information

How to Cite

Trincado, D. E., Scott, G. M., White, P. A., Hunt, C., Rasmussen, L. and Rawlinson, W. D. (2000), Human cytomegalovirus strains associated with congenital and perinatal infections . Journal of Medical Virology, 61: 481–487. doi: 10.1002/1096-9071(200008)61:4<481::AID-JMV11>3.0.CO;2-H

Author Information

  1. 1

    Virology Division, Department of Microbiology, South Eastern Area Laboratory Services, The Prince of Wales Hospital, Randwick, Australia

  2. 2

    School of Pathology, University of New South Wales, Kensington, Australia

  3. 3

    School of Science, University of Western Sydney, Nepean, Kingswood, Australia

  4. 4

    Stanford University School of Medicine, Center for AIDS Research and Division of Infectious Diseases and Geographic Medicine, Stanford, California

*Virology Division, Department of Microbiology, South Eastern Area Laboratory Services, The Prince of Wales Hospital, Randwick NSW 2031, Australia

  1. †

    This work was performed at the Virology Division, Department of Microbiology, South Eastern Area Laboratory Services, The Prince of Wales Hospital, Randwick, Australia.

Publication History

  1. Issue published online: 10 JUL 2000
  2. Article first published online: 10 JUL 2000
  3. Manuscript Accepted: 16 DEC 1999

Funded by

  • National Health and Medical Research Council
  • University of Western Sydney-Seed Grant
  • Sydney Children's Hospital Foundation

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Keywords:

  • glycoprotein B;
  • polymerase chain reaction;
  • restriction fragment length polymorphism;
  • single-stranded conformation polymorphism;
  • heteroduplex mobility analysis

Abstract

The genotypes of human cytomegalovirus (HCMV) isolates from pediatric patients differs from those of infected adults in Australia. Genotypes were determined by PCR amplification of glycoprotein B (gB) sequences, with subsequent analysis by restriction fragment length polymorphism, single-stranded conformation polymorphism, heteroduplex mobility analysis and direct DNA sequencing. Restriction fragment length polymorphism analysis of gB showed genotypes gB1 (39%) and gB3 (30%) were more prevalent in infected children and two new genotypes (gB6 and gB7) were found. Single-stranded conformation polymorphism was used to group isolates into 22 further subtypes and suggested longitudinal co-infection or viral mutation was occurring over time. Heteroduplex mobility analysis was found to be the most accurate and concise of the four methods used for genotyping HCMV isolates. DNA sequencing was used to confirm the results obtained from heteroduplex mobility analysis, and identified two isolates that were incorrectly genotyped by restriction fragment length polymorphism analysis. Heteroduplex mobility analysis efficiently genotyped all samples and allowed estimation of sequence variation between isolates. These data suggest certain gB genotypes are associated more commonly with childhood infections, and these differ from strains associated with invasive disease in HIV patients. J. Med. Virol. 61:481–487, 2000. © 2000 Wiley-Liss, Inc.

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