Yeast Functional Analysis Report
Rapid and reliable protein extraction from yeast
Article first published online: 7 JUN 2000
Copyright © 2000 John Wiley & Sons, Ltd.
Volume 16, Issue 9, pages 857–860, 30 June 2000
How to Cite
Kushnirov, V. V. (2000), Rapid and reliable protein extraction from yeast. Yeast, 16: 857–860. doi: 10.1002/1097-0061(20000630)16:9<857::AID-YEA561>3.0.CO;2-B
- Issue published online: 7 JUN 2000
- Article first published online: 7 JUN 2000
- Manuscript Accepted: 14 FEB 2000
- Manuscript Received: 29 JUL 1999
- Wellcome Trust Collaborative Research Initiative, UK
- protein extraction;
The methods currently used for protein extraction from yeast are either laborious or insufficiently reliable. Here I report a method for protein extraction for electrophoretic analysis that is both easy and reliable. In this method, yeast cells are subjected to mild alkali treatment and then boiled in a standard electrophoresis loading buffer. The method was tested for different strains of Saccharomyces cerevisiae and for yeast Hansenula polymorpha DL-1. It yields virtually complete extraction independently of the strain, growth conditions and protein molecular weight and allows working with small amounts of yeast cells grown on agar plates. Copyright © 2000 John Wiley & Sons, Ltd.