Rapid and reliable protein extraction from yeast

Authors

  • Vitaly V. Kushnirov

    Corresponding author
    1. Institute of Experimental Cardiology, Cardiology Research Centre, Moscow, Russia
    • Institute of Experimental Cardiology, Cardiology Research Centre, 3rd Cherepkovskaya Street 15A, 121552 Moscow, Russia.
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Abstract

The methods currently used for protein extraction from yeast are either laborious or insufficiently reliable. Here I report a method for protein extraction for electrophoretic analysis that is both easy and reliable. In this method, yeast cells are subjected to mild alkali treatment and then boiled in a standard electrophoresis loading buffer. The method was tested for different strains of Saccharomyces cerevisiae and for yeast Hansenula polymorpha DL-1. It yields virtually complete extraction independently of the strain, growth conditions and protein molecular weight and allows working with small amounts of yeast cells grown on agar plates. Copyright © 2000 John Wiley & Sons, Ltd.

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